Coding

Part:BBa_K1985005

Designed by: Jasmine Cornish   Group: iGEM16_Kent   (2016-10-14)
Revision as of 13:02, 14 October 2016 by Registry (Talk | contribs)

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mamX his-tagged, signal sequence cleaved

Usage: The mam gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the protein is exposed to into in solution. This sequence produces a soluble protein because it has the membrane anchor cleaved and is targeted to the secretory pathway, which will leave the protein in the periplasm. It was expressed, purified and then exposed to iron. Biology: Mam is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 216
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 783


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Categories
Parameters
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