Generator

Part:BBa_K2012014

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)
Revision as of 13:48, 6 October 2016 by Registry (Talk | contribs)

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A medium CheZ generator with fusion GFP reporter

This generator contain promotor J23106 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate medium concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promotor and its swimming ability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


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Categories
Parameters
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