Generator

Part:BBa_K2012013

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)
Revision as of 10:49, 6 October 2016 by Registry (Talk | contribs)

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Strong CheZ generator with a fusion GFP reporter

This generator contain promotor J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate CheZ with a high ouIt is used to detect the relationship of CheZ under different promotor strength and its swimming ability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


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Categories
Parameters
None