Composite

Part:BBa_K1871010

Designed by: Broad Run High School iGEM Team   Group: iGEM15_BroadRun-NorthernVA   (2015-09-13)
Revision as of 00:56, 17 September 2015 by Nickrozak33 (Talk | contribs) (→‎Alpha Amylase)

Coding Sequence AMY1

Alpha amylase protein


This is an alpha amylase gene obtained from Bacillus amyloliquefaciens. It is flanked by a Kozak sequence and ADH1 terminator but lacks a promoter.

Kozak Sequence

This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

Native Alpha Amylase Secretion Sequence

This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1436
    Illegal NgoMIV site found at 1451
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None