Translational_Unit

Part:BBa_K2042012

Designed by: Delphine Dessaux   Group: iGEM16_Evry   (2016-09-03)
Revision as of 21:49, 15 October 2016 by RATOVOMANANA (Talk | contribs)


RBS + PhaC_sp. MBEL 6-19

This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick BBa_K2042014 BBa_K2042014

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None