Part:BBa_K2012007
CheZ fussed with a CG linker and reporter GFP(BBa_E0040)
the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly.<\br>
cheZ gene, regulating the bacterial motility by decreasing the possibility of tumbling, is a chemotaxis relating gene, It is wildly used in iGEM to control the motility of bacteria. In igem 2016 HZAU-China's project, we successfully fused a reporter protein GFP(BBa_E0040) with CheZ through a 18bp GS linker to monitor the concentration of CheZ.
We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1301
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