Part:BBa_K1884009

PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

The device we constructed this year is one of the functional plasmids in the light-mediated expression system,followed the example of yeast two-hybrid system. With BD-CIB1 which is constructed in the other device(BBa_K1884007), light-mediated expression system, followed the example of yeast two-hybrid system, will be activated and starting to transcript Ferredoxin-NADP(+)Reductase(BBa_K1884006) which is on the 3 prime end of Upstream activating sequence(BBa_K1884004) in green algae

Biology

PASD promoter(BBa_K1547005) is a plant promoter from the green algae Chlamydomonas reinhardtii. It is a high expression promoter that encodes for a ferrodoxin-binding protein of photosystem I.This year we decided to built the light-mediate-controlled system in green algae Chlamydomonas reinhardtii.Based on the origin of PSAD promoter, we thought it would be fully competible in our project.

AD-CRY2

UAS

FNR

PSAD Terminator

Usage

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3123
  Illegal NotI site found at 3221
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1560
  Illegal BglII site found at 2019
  Illegal BamHI site found at 2498
  Illegal XhoI site found at 3052
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1444
  Illegal AgeI site found at 2173
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1796
  Illegal BsaI.rc site found at 1205
  Illegal SapI.rc site found at 1313
  Illegal SapI.rc site found at 3384