![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100009
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-10)
pENTR pPRE4
The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 19
Illegal BsaI.rc site found at 265
[edit]
Categories
Parameters
//awards/part_collection/2016
None |