![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100005
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-10)
pENTR eYFP
eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 222
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 222
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 222
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 222
Illegal AgeI site found at 5 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/part_collection/2016
None |