Coding
ecf16

Part:BBa_K2070001

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)
Revision as of 01:02, 15 October 2016 by RinaSakata (Talk | contribs)


ecf16

Ecf16 is an extracytoplasmic function (ECF) sigma factor taken from Pseudomonas entomophila used to activate the ecf16 promoter (BBa_K2070000). The sigma factor is found to be highly orthogonal to its corresponding promoter and inactivated by antisigma factor16 (No part name specified with partinfo tag.).

Source

Pseudomonas entomophila L48

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None