Composite

Part:BBa_K2016005

Designed by: Magdalena Dabrowska   Group: iGEM16_Sheffield   (2016-10-07)
Revision as of 20:22, 14 October 2016 by Dvdhrnby (Talk | contribs) (→‎Usage and Biology)


Superfolder GFP under control of strong constitutive promoter and RyhB siRNA domain

Usage and Biology

This part consists of the superfolder GFP gene (sfgfp), with a RhyB binding sequence in its 5' UTR. RhyB is an srRNA that is expressed in E.coli and several other bacteria under low iron conditions. This results in sfGFP being downregulated in low iron conditions, which we see in the form of lower florescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 38


Functional Parameters

[edit]
Categories
//function/reporter/fluorescence
Parameters
None