Part:BBa_K1937008
AF-A antifungal operon in pSBBs0K-mini
(Chassis B. subtilis, carrier plasmid pSBBS0K) Length: 6558bp Background: AF-A is a part designed to be combined with AF-B to form an operon of 5 antifungal peptides. The interest of using several antifungals is to have a broad spectrum of fungi target, because each antifungal has its own characteristics.The peptides were designed to be expressed and secreted by Bacillus subtilis. This operon was also initially designed to be expressed under the control of the pNagA or pNagP promoter (BBa_K1937003 and BBa_K1937005 respectively). In spite of several cloning strategies, the AF-B construction was not obtained, likely due to the toxicity of one of the peptides for Bacillus subtilis. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1228
Illegal suffix found in sequence at 1256 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1228
Illegal NheI site found at 250
Illegal NheI site found at 1250
Illegal SpeI site found at 1257
Illegal PstI site found at 1271
Illegal NotI site found at 1234
Illegal NotI site found at 1264 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1228
Illegal BglII site found at 970
Illegal BglII site found at 5785
Illegal BamHI site found at 1301 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1228
Illegal suffix found in sequence at 1257 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1228
Illegal XbaI site found at 1243
Illegal SpeI site found at 1257
Illegal PstI site found at 1271
Illegal NgoMIV site found at 2848
Illegal AgeI site found at 5396
Illegal AgeI site found at 6358 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 438
Illegal BsaI.rc site found at 1026
Illegal BsaI.rc site found at 2672
Illegal BsaI.rc site found at 4111
Illegal BsaI.rc site found at 6627
Illegal SapI site found at 1589
Illegal SapI.rc site found at 5609
None |