Coding

Part:BBa_I718002:Design

Designed by: David Puyraimond   Group: iGEM07_Paris   (2007-07-10)
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Diacylglycerol-Acyl-transferase (DGAT) for triglyceride synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutagenized Pst-1 site in position 1062 after ATG. We mutagenized stop codon TAAAAA into TAATAA to conform with BioBrick standards. We added in 5' a strong RBS (BBa_B0030) and prefixe biobrick (BBa_G00000). In 3', we added suffixe biobrick (BBa_G00001).


Source

  • Acinetobacter calcoaceticus ADP1
  • Accession number: AF529086
  • Kalscheuer,R. and Steinbuchel,A. A Novel Bifunctional Wax Ester Synthase/Acyl-CoA:Diacylglycerol Acyltransferase Mediates Wax Ester and Triacylglycerol Biosynthesis in Acinetobacter calcoaceticus ADP1. J. Biol. Chem. 278 (10), 8075-8082 (2003)

References