Protein_Domain

Part:BBa_K1935018:Design

Designed by: Emilie Gounin   Group: iGEM16_Bordeaux   (2016-10-14)
Revision as of 14:54, 14 October 2016 by EmiG (Talk | contribs) (→‎Design Notes)


dead Cas9 (dCas9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 2182
    Illegal NgoMIV site found at 2255
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 3649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis was done to abolish illegal restriction site (Pst1)

Source

https://www.addgene.org/47106/sequences/

References