Coding
Part:BBa_K1981007
Designed by: Yulei Dang Group: iGEM16_NKU_China (2016-10-14)
mtn
S-ribosylhomocysteine lyase. Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 475
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The AI-2 “supplier” was constructed by overexpressing the luxS gene.
This part was originated from Bacillus amyloliquefaciens LL3.
Characterization
Protein-Data Table
[edit]
Categories
Parameters
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