Composite

Part:BBa_K1918104:Design

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:28, 14 October 2016 by SheepW (Talk | contribs) (→‎Design Notes)


TRE-Kozak-IntC:EBFP2C(155-238)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AIM: the expression of IntC:EBFP2C(155-238) could be induced by Dox; ELEMENTS: IntC, EBFP2C(155-238), Dox-induced promoter TRE

Source

PCR from the existing plasmid in xie lab

References