Composite

Part:BBa_K1918106:Experience

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 07:46, 14 October 2016 by SheepW (Talk | contribs) (Applications of BBa_K1918106)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1918106

1. Use PCR to get the DNA fragment of CMV and EBFP2N(1-154):IntN 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. FACS

User Reviews

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