Composite
Part:BBa_K1918102:Experience
Designed by: Linghan Wang Group: iGEM16_Tsinghua-A (2016-10-14)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1918102
1. Use PCR to get the DNA fragment of TRE and EBFP2C(155-238) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of EBFP2C(155-238) 10. FACS
User Reviews
UNIQa82b647ee26bc0cf-partinfo-00000000-QINU UNIQa82b647ee26bc0cf-partinfo-00000001-QINU