Device

Part:BBa_K1919300

Designed by: Shaobo Yang   Group: iGEM16_SCU-China   (2016-09-16)
Revision as of 06:49, 16 September 2016 by Registry (Talk | contribs)

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livJ

This part contains a promoter, RBS, protein coading region and a terminator. The expression of the protein livJ in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.

LivJ is a crutial subunit of leucine transporter which is regulated by xxx. Overexpression of livJ can increase the untilizaiton of leucine in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 203
    Illegal AgeI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 366


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Categories
Parameters
None