Device
Part:BBa_K1919300
Designed by: Shaobo Yang Group: iGEM16_SCU-China (2016-09-16)
livJ
This part contains a promoter, RBS, protein coading region and a terminator. The expression of the protein livJ in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.
LivJ is a crutial subunit of leucine transporter which is regulated by xxx. Overexpression of livJ can increase the untilizaiton of leucine in E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 382
Illegal AgeI site found at 203
Illegal AgeI site found at 485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 366
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Categories
Parameters
None |