Part:BBa_K2139001
Coding Sequence of Endo5a
BBa_K2139000 encompass the coding region for an endo-beta-1,4-glucanase known as Endo5A. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and is codon optimized to contain a high GC content.
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 975
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 406
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 303
None |