Coding

Part:BBa_K2139004

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
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Coding Sequence of SLAC

BBA_K2139004 is the coding region of SLAC (Small LACcase). It is a small 229 amino acid protein requiring four copper ions per monomer as cofactors. Its function is to oxidize a range of substrates, including phenols, aromatic amines, and nonphenolic substrates involved in lignol degradation. The GenBank number is of a protein homolog (single insertion of Lysine at amino acid 220)is WP_020416648 (amino acid) with a PDB of 3T9W_A.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 41
    Illegal XhoI site found at 130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None