Composite

Part:BBa_K2089006

Designed by: Rouxian Zeng   Group: iGEM16_GDSYZX-United   (2016-10-12)
Revision as of 05:14, 12 October 2016 by Registry (Talk | contribs)

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pHHL-hhl1

This biobrick consists of a complete functional protein HHL1 coding sequence in Arabidopsis and gene hhl1.Gene hhl1 encodes protein HHL1,which is produced and distributed among chloroplasts(especailly grana core-,grana margin and stroma lamellae enriched thylokoid membranes) to function in protecting and repairing photosystemⅡfrom photodamage. pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 746
    Illegal PstI site found at 2352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 187
    Illegal SpeI site found at 746
    Illegal PstI site found at 2352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 746
    Illegal PstI site found at 2352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 746
    Illegal PstI site found at 2352
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None