Composite

Part:BBa_K1997015:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 10:06, 13 October 2016 by Registry (Talk | contribs)

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P+R->sGFP-N->Zif268->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1003
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1217


Design Notes

This part is designed to be a positive control which contains the complete ZFN protein. Compared with the experimental groups, it will be easily to find the disparity between them.


Source

We obtained it through the contribution of our basic subparts.

References