RNA
Part:BBa_K2020042:Experience
Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath Group: iGEM16_Aachen (2016-10-02)
Revision as of 13:51, 2 October 2016 by Andrea hoeltken (Talk | contribs)
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Applications of BBa_K2020042
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UNIQ56c5e27ff12ca93b-partinfo-00000000-QINU UNIQ56c5e27ff12ca93b-partinfo-00000001-QINU
Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.
[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.