Part:BBa_K1968002

Synechocystis RBS* Phytobrick

RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034.

Comparison of ribosome binding sites BBa_B0030, BBa_B0032, BBa_B0034 and RBS* in Escherichia coli DH5α (white bars) and Synechocystis PCC 6803 (black bars). The translational efficiencies were measured by means of GFP fluorescence and divided by the absorbance of the cultures at 595 nm (E. coli) or 750 nm (Synechocystis), respectively. The data represent mean ± S.D. of 4 measurements each of three independent cultivations (1).

(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]