Coding

Part:BBa_K2148013:Design

Designed by: Chern Juin Ong, Alex Mayorov, Mie Monti   Group: iGEM16_Cambridge-JIC   (2016-10-11)
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Cas9 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Gly-Ser linker is advised by Dr N. Patron (Earlham Institute)


Source

Streptococcus pyogenes

References