Regulatory

Part:BBa_K2148017

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-13)
Revision as of 19:47, 13 October 2016 by Registry (Talk | contribs)

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C.reinhardtii chloroplast rbcL 3UTR w/ Homologies

This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome and has the homology regions right from the trnE2-psbH integenic region with psaA promoter. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation, which would also be available for Homologous Recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 515
    Illegal NheI site found at 1944
    Illegal NheI site found at 2265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 77
    Illegal BsaI.rc site found at 2670


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Categories
Parameters
None