Composite

Part:BBa_K2120311

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 18:49, 13 October 2016 by YangHongYu (Talk | contribs)


B0015+tetR+B0034+pBAD-AraC+B0015+ptet+B0034+RFP+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of ptet promoter. We use it to control the “on” or ”off” state of toxin gene. RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120303】 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 911
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1076
    Illegal AgeI site found at 2839
    Illegal AgeI site found at 2951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1093


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Categories
Parameters
None