Coding

Part:BBa_K1957008

Designed by: Nancy Teng   Group: iGEM16_NRP-UEA-Norwich   (2016-10-13)
Revision as of 17:47, 13 October 2016 by Registry (Talk | contribs)

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HyaB N-terminally tagged subunit of NiFe Hydrogenase

One of the three subunits that make up NiFe Hydrogenase in Shewanella oneidensis. Specifically this is the HyaB subunit, which is the membrane-bound subunit. This subunit has a strep-II tag on its CN-terminal for downstream protein assays. This gene encodes one out of the three subunits that can be used to make the entire NiFe hydrogenase construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 282
    Illegal BamHI site found at 1479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1766
    Illegal SapI.rc site found at 37


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Categories
Parameters
None