Coding

Part:BBa_K1896007

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)
Revision as of 17:52, 13 October 2016 by Registry (Talk | contribs)

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mGFPuv2 (C-part)

Variant of Part:BBa_K1896001 where the start codon was replaced by a GSTGS linker so it can be used in C-terminal protein fusions.

mGFPuv2 contains the following mutations:

  • F99S/M153T/V163A: GFPuv or cycle 3 GFP was optimised for excitation by UV light (360-400nm). [1]
  • S208L: Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2]
  • A206K: Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None