Coding

Part:BBa_K1641003

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-08)
Revision as of 09:20, 8 September 2015 by Registry (Talk | contribs)

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Recombinase Vika, E. coli and yeast coding optimized

This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system.

Vika has the invertase activity that invert the the sequence between two anti-parallel Voxs. The original Vika is discovered through genome screening of Vibrio coralliilyticus ATCC BAA-450, which might be a integrase gene taken in by some phage. Yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to original protein sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None