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Coding
Part:BBa_K1641003
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-08)
Recombinase Vika, E. coli and yeast coding optimized
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system.
Vika has the invertase activity that invert the the sequence between two anti-parallel Voxs. The original Vika is discovered through genome screening of Vibrio coralliilyticus ATCC BAA-450, which might be a integrase gene taken in by some phage. Yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to original protein sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |