Coding

Part:BBa_K1896004

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)
Revision as of 09:39, 13 October 2016 by Registry (Talk | contribs)

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linker-Streptavidin-His (tetrameric)

This is an improved version of Part:BBa_K283010, which encodes for the core Streptavidin protein of Streptomyces avidinii. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None