RNA

Part:BBa_K1942000:Experience

Designed by: Chai Yingzi, Chen Jingyi, Deng Chenghui, Gu Xiaoyang, Peng Yue   Group: iGEM16_NJU-China   (2016-10-10)
Revision as of 08:10, 13 October 2016 by I (Talk | contribs) (→‎Applications of BBa_K1942000)


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Applications of BBa_K1942000

USAGE AND BIOLOGY

We package KRAS siRNA into exosomes by transfecting HEK293 cells with a plasmid expressing KRAS siRNA and then collect siRNA-encapsulated exosomes. When inject the modified exosomes into the vein, exosome will specifically recognize integrin receptors and fuse with Lung adenocarcinoma cells under the direction of the iRGD peptide. Once inside cells, KRAS siRNA will degrade KRAS mRNA by base-pairing, resulting in sharp decrease of K-ras in Lung cancer cells. As a consequence, K-ras reduction and disturbed function will result in the inhabitation of the proliferation of cancer cells, which ultimately have some therapeutic effects on Lung cancer.

NJU China 2016 iGEM Parts Description K1942000 Figure 2.png

User Reviews

UNIQ327e0ee7cc1a9a68-partinfo-00000000-QINU UNIQ327e0ee7cc1a9a68-partinfo-00000001-QINU