Coding

Part:BBa_K2132001

Designed by: Shijie Gu   Group: iGEM16_ShanghaitechChina   (2016-08-26)
Revision as of 07:59, 13 October 2016 by LechenQian (Talk | contribs)


CsgASpyCatcherHisTag

This is the subunit of the biofilm of E. Coli Curli system linked to SpyCatcher and HisTag. The two added parts can be used for various functions with SpyTag and other His tag-binding materials like quantum dots.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

TEM: visualization of CsgASpyCatcherHisTag mutant biofilm

[edit]
Categories
Parameters
None