Coding

Part:BBa_K2019003

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-10-12)
Revision as of 02:28, 13 October 2016 by Registry (Talk | contribs)

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Periplasm Directed saCas9 via TorA Signaling Sequence (TorA-saCas9)

TorA-Cas9 is a fusion of the TorA signaling sequence to the N-terminus of staphylococcus aureus CRISPR associated protein 9 (saCas9). TorA utilizes the twin arginine translocation (tat) pathway to direct the fused protein to the periplasm of the cell. Therefore, by fusing Cas9 to the C-terminus of the signaling sequence, it should be directed to the periplasm of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 753
    Illegal BglII site found at 906
    Illegal BglII site found at 1052
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal AgeI site found at 2882
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None