Coding

Part:BBa_K2019000

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-07-15)
Revision as of 16:39, 15 July 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Histidine Tagged WT Staphylococcus Aureus Cas9 Protein

Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli. saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as it the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9. Furthermore, saCas9 has been shown to cut just as efficiently as spCas9 while also offering a more specific PAM sequence of NNGRRT. The his tag (his6) was attached to saCas9 to allow one to able to run a FPLC test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 627
    Illegal BglII site found at 780
    Illegal BglII site found at 926
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal AgeI site found at 2756
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None