Composite

Part:BBa_K2042014

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-10-04)
Revision as of 21:59, 12 October 2016 by RATOVOMANANA (Talk | contribs)


RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)

This is the sequence of the PLA producing operon. This sequence is optimized for P.putida and is constituted of two gene propionate CoA transferase (Pct) and PHA synthase (PhaC). Pct contain a mutation that allows him to take lactate as the acceptor and convert it efficiently into lactyl-CoA. The PhaC gene contains 4 amino acid mutation which were found to polymerize monomer of lactyl-coA into PLA homopolymer. This gene is placed upstream in the sequence closer of the promoter because polymerization is the bottleneck of the PLA producing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2792
    Illegal BsaI site found at 3263


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None