Coding
Csp1sfGFP

Part:BBa_K1980001:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 13:55, 11 October 2016 by Registry (Talk | contribs)

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TAT Copper Storage Protein 1 sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.


Source

The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.

References