Device

Part:BBa_K1919001

Designed by: Shiji Zhao   Group: iGEM16_SCU-China   (2016-09-05)
Revision as of 08:56, 5 September 2016 by Registry (Talk | contribs)

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Constitutive promotor J23105 and downstream CecropinXJ

Biobrick BBa_K1919001 is a composite part, consisting of constitutive promotor J23105, RBS, TrxA tag, 6xHis tag, thrombin site, S tag, enterkinase site and the coding sequence of antimicrobial peptide CecropinXJ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 506
    Illegal BamHI site found at 549
    Illegal XhoI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/odor
Parameters
biologyBombyx mori
chassisE.coli
proteinTrxA+6xHis tag+Thrombin site+S Tag+enterokinase site+CecropinXJ