Coding

Part:BBa_K2114001

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-06)
Revision as of 11:28, 11 October 2016 by WladStr (Talk | contribs) (→‎Usage and Biology)


aGFPnano_HA_aHelix_cotZ

N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ.


Usage and Biology

This part includes the anti-GFP nanobody (describted by Kubala et al. [Ref]) fused by an alpha helical linker [Ref] to the B. subtilis spore crust protein CotZ in order to be displayed on the spore surface. The hemagglutinin epitope tag was included in the fusion construct for convenient detection by specific anti-HA antibodies.

[Figure of construct]

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 465
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None