Coding

Part:BBa_K2148001

Designed by: Ciara McCarthy   Group: iGEM16_Cambridge-JIC   (2016-08-12)
Revision as of 23:14, 9 October 2016 by Cjong (Talk | contribs) (→‎Usage and Biology)


aadA

A commonly used antibiotic for Chlamydomonas chloroplasts. Confers resistance to spectinomycin and streptomycin


Usage and Biology

The aadA gene confers antibiotic resistance to Spectinomycin and Streptomycin.

This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

References

Goldschmidt-Clermont M. Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker of site-directed transformation of chlamydomonas. Nucleic Acids Research. 1991;19(15):4083-4089.

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