Project

Part:BBa_K1926003:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 04:17, 11 October 2016 by CindyLiu (Talk | contribs) (→‎References)


A cyclic promoter of CDK4 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 522
    Illegal NheI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got the sequence from human genome through PCR using the following primers:

pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC

pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA

(Product: 457)

Source

The sequence was retrieved from Addgene.

References

1. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.