Project

Part:BBa_K1926002:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 04:40, 10 October 2016 by Registry (Talk | contribs)

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A cyclic promoter of Ki-67 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 422


Design Notes

Mutation to remove Pst1


Source

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pKi67-F: ACCTCTGCCCTCCGCCAGCCG pKi67-R: ACCCGGTGGCCCTACAGGCTACG (Product: 360)


References