Project

Part:BBa_K1926001:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 02:53, 11 October 2016 by CindyLiu (Talk | contribs) (→‎Design Notes)


A cyclic promoter of Cyclin E from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 382
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
    Illegal XhoI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 491
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got this sequence from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)

Source

The sequence was retrieved from Addgene.

References