Regulatory

Part:BBa_K2148004

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-09)
Revision as of 23:33, 9 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Homology-psaA promoter/5UTR/NTAG

A level-0 Phytobrick coded as a promoter/5UTR with a Chlamydomonas reinhardtii chloroplast homology region upstream of the promoter sequence to allow for homologous recombination. The homology region is left from the trnE2-psbH integenic region in the Chlamydomonas reinhardtii chloroplast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None