Regulatory
Part:BBa_K2148004
Designed by: Chern Juin Ong Group: iGEM16_Cambridge-JIC (2016-10-09)
Homology-psaA promoter/5UTR/NTAG
A level-0 Phytobrick coded as a promoter/5UTR with a Chlamydomonas reinhardtii chloroplast homology region upstream of the promoter sequence to allow for homologous recombination. The homology region is left from the trnE2-psbH integenic region in the Chlamydomonas reinhardtii chloroplast.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4
Illegal XbaI site found at 50 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4
Illegal XbaI site found at 50 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4
Illegal XbaI site found at 50 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |