Part:BBa_K1897002

HasR Membrane receptor coding sequence

HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1866
  Illegal NotI site found at 2660
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 24
  Illegal NgoMIV site found at 28
  Illegal NgoMIV site found at 84
  Illegal NgoMIV site found at 202
  Illegal NgoMIV site found at 356
  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 757
  
• 1000
  COMPATIBLE WITH RFC[1000]