Device

Part:BBa_K2008001

Designed by: Rachelle Varga   Group: iGEM16_UofC_Calgary   (2016-10-08)
Revision as of 21:38, 8 October 2016 by Rcvarga (Talk | contribs)


BBI with N-terminal KSCI solubility tag fused to GFP

This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. This component of the composite part is fused to the N-terminal end of GFP (BBa_E0040) for visual detection, and is under the control of a constitutive E. coli promoter (BBa_J2310) and strong RBS (BBa_B0034).

Note: This part is a composite part, not a basic part, but it could not be entered as composite at this time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742


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Categories
Parameters
None