Coding

Part:BBa_K2132004:Design

Designed by: YIFAN CHEN   Group: iGEM16_ShanghaitechChina   (2016-10-09)
Revision as of 11:25, 9 October 2016 by Chenyf (Talk | contribs) (→‎Source)


HydA with SpyCatcher, Histag and TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
    Illegal AgeI site found at 1300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2053
    Illegal SapI.rc site found at 663
    Illegal SapI.rc site found at 1005


Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.