Coding

Part:BBa_K2010004

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)
Revision as of 17:55, 21 June 2016 by Registry (Talk | contribs)

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sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)

This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 743
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1161


[edit]
Categories
//function/degradation
Parameters
protein