Coding

Part:BBa_K2010003:Design

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)
Revision as of 17:50, 21 June 2016 by Registry (Talk | contribs)

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YebF + PETase (PET-degrading enzyme, origin I. sakaiensis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 280
    Illegal SapI site found at 816


Design Notes

The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.


Source

This sequence is publicly known and was privately modified.

References